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| Silva-Pinhati,Ana C.O.; Bacci Jr.,Maurício; Siqueira,Célia G.; Silva,Aline; Pagnocca,Fernando C.; Bueno,Odair C.; Hebling,Maria J.A.. |
| The isolation and maintenance of symbiotic basidiomycete fungi living in association with ants of the tribe Attini has been hindered by the slow growth rate of these fungi and the presence of other microorganisms on the surface of the material which the ants maintain inside their nests to provide a growth substrate for their symbiont. In this paper we describe a method which increases the efficiency of isolation of these fungal symbionts by over seven fold as compared to traditional isolation procedures. Underground nests of attine ants of the genera Atta, Acromyrmex, Trachymyrmex and Mycetarotes were located, from which samples containing the fungal symbiont and ants were collected and transported to the laboratory where the ants were able to clean the... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Atta; Acromyrmex; Trachymyrmex; Mycetarotes; Basidiomycete; Symbiosis. |
| Ano: 2005 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1519-566X2005000100001 |
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| Pereira,Daniela Isabel Brayer; Botton,Sônia de Avila; Azevedo,Maria Isabel de; Monteiro,Danieli Urach; Weiblen,Carla; Machado,Vanessa Schopf; Donatti,Michele Guimarães; Loeck,Alci Enimar. |
| Leaf-cutting ants of the genera Atta and Acromyrmex determine serious agricultural problems and live on symbiosis with Leucoagaricus gongylophorus. The aim of this study is to identify morphological and molecularly, as well as to verify the genotypic variability of the symbiotic fungus cultivated by A. heyeri and A. ambiguus from three different regions of the state of Rio Grande do Sul, Brazil. Fungus gardens were collected and fragments of mycelia were grown in selective medium. Total DNA was extracted and amplification of the ITS region was performed by PCR using universal primers. After DNA sequencing, the chromatograms were assembled and phylogenetic analyzes were performed by the Neighbor-Joining method. A total of six isolates of L. gongylophorus... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Basidiomycete; Leucoagaricus gongylophorus; Internal transcribed spacer.. |
| Ano: 2015 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782015000701256 |
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| Galicia-García,Porfirio Raúl; Silva-Rojas,Hilda Victoria; Mendoza-Onofre,Leopoldo Ernesto; Zavaleta-Mancera,Hilda Araceli; Córdova-Téllez,Leobigildo; Espinosa-Calderón,Alejandro. |
| ABSTRACT Ustilago maydis is a basidiomycete known as the causative agent of 'common smut', worldwide disease of maize that is recognized by the galls it forms, which have considerable potential as a gourmet food. Results of infection are quite variable, even under optimal greenhouse conditions. In order to find pathogenic strains able to be used as a highly infective and stable inoculum for the successful production of galls either in greenhouses or in the field, ears with gall symptoms containing teliospores were recovered from maize plants. The teliospores were suspended in water and plated on nutrient-rich medium. Twenty-six colonies developed, containing three types of yeast-like colonies: saprotrophic, pathogenic, and solopathogenic. DAPI staining... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Basidiomycete; Maize galls; Mating types; Molecular diagnosis; Smut. |
| Ano: 2016 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-33062016000400683 |
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